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This study focuses on the evaluation of current water quality and ecotoxicology techniques for the metal assessment of an upland limestone catchment located within a historical metal (lead ore) mining area in northern England. Within this catchment, metal toxicity occurs at circumneutral pH (6.2 7.5). Environmental Quality Standards (EQSs) based on a simple single concentration approach like hardness based EQS (EQS H) are more overprotective, and from sixteen sites monitored in this study more than twelve sites (>75%) failed the EQSs for Zn and Pb.
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The main aims of this study were to investigate the molecular mechanisms underlying TG2 modulation by PACAP and NGF and its role in neurite outgrowth and neuroprotection in differentiating mouse N2a and human SHSY5Y neuroblastoma cells.In the first part, the regulation of TG2 transamidase activity by PACAP 27 and NGF in differentiating mouse N2a and human SH SY5Y neuroblastoma cells was assessed. TG2 transamidase activity was determined using an amine incorporation and a peptide cross linking assay. In situ TG2 activity was assessed by visualising the incorporation of iotin X cadaverine using confocal microscopy.
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